The Work Organisation Assessment questionnaire (WOAq) has been translated into Italian, back-translated, and modified. Data were collected from 160 health care workers and 50 white-collar bank employees. Employee wellbeing was assessed by the General Health Questionnaire (GHQ12), while work stress was assessed by the Karasek's Job Content Questionnaire (JCQ). Reliability, as measured by Cronbach's alpha, was very good (0.95). Principal component analysis revealed that a significant percentage of the variance (41.8%) was explained by a single summative factor which included 25 of the 28 items. Varimax orthogonal rotation yielded the same five factors observed in the original questionnaire (reward and recognition, quality of relationship with management, quality of physical environment, quality of relationship with colleagues, workload). The WOAQ sum score was positively related to social support, as measured by the Karasek's JCQ (Spearman's rho = 0.523; p < 0.001). It was negatively related both to job strain (Spearman's rho = -0.516; p < 0.001) and psychological distress GHQ (Spearman's rho = -0.365; p < 0.001). In conclusion, the Italian version of WOA maintains the original characteristics. The questionnaire has strong association with job stress and employee wellbeing, and it may be useful in risk assessment procedures.