Measurement of interleukin-21

Curr Protoc Immunol. 2007 Aug:Chapter 6:6.30.1-6.30.8. doi: 10.1002/0471142735.im0630s78.

Abstract

This unit describes three procedures for measurement of interleukin-21 (IL-21). The first employs the use of an antibody sandwich ELISA. An alternative procedure measures proliferative responses of T cells to a combination of IL-21 and IL-15 using CFSE. Finally, a method to assess IL-21-induced tyrosine phosphorylation of Stat3 in splenic CD8(+) T cells using a flow cytometry-based analysis is described.

MeSH terms

  • Animals
  • Antibodies, Monoclonal
  • Antibodies, Phospho-Specific / immunology
  • Antibodies, Phospho-Specific / metabolism
  • CD8-Positive T-Lymphocytes
  • Cell Separation / methods
  • Enzyme-Linked Immunosorbent Assay
  • Flow Cytometry / methods
  • Fluoresceins / metabolism
  • Immunologic Techniques*
  • Immunologic Tests / methods
  • Interleukin-15 / immunology
  • Interleukin-15 / metabolism
  • Interleukins / analysis*
  • Interleukins / immunology
  • Interleukins / metabolism
  • Lymphocyte Activation
  • Mice
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • STAT3 Transcription Factor / immunology
  • STAT3 Transcription Factor / metabolism
  • Succinimides / immunology
  • Succinimides / metabolism

Substances

  • 5-(6)-carboxyfluorescein diacetate succinimidyl ester
  • Antibodies, Monoclonal
  • Antibodies, Phospho-Specific
  • Fluoresceins
  • Interleukin-15
  • Interleukins
  • Recombinant Proteins
  • STAT3 Transcription Factor
  • Succinimides
  • interleukin-21