Association of the CALM1 core promoter polymorphism with knee osteoarthritis in patients of Greek origin

Genet Test. 2008 Jun;12(2):263-5. doi: 10.1089/gte.2007.0114.

Abstract

Purpose: Osteoarthritis (OA) is characterized by focal areas of loss of articular cartilage in synovial joints, associated with varying degrees of osteophyte formation, subchondral bone change, and synovitis. The Calmodulin 1 gene (CALM1) encodes for a ubiquitous eukaryotic Ca2+ binding protein and is the principal mediator of the calcium signal. The protein thus affects the chondrocyte's response to mechanical load. A functional core promoter single nucleotide polymorphism (SNP) -16C/T (rs12885713) was recently associated with hip OA (HOA) in the Japanese population, while no association was found in a British group of patients with HOA. Our objective was to assess whether this SNP was also associated with knee OA (KOA) in a Greek Caucasian population sample.

Methods: The -16T/C SNP was genotyped in a 351 case-control cohort--158 patients with idiopathic KOA and 193 controls.

Results: No significant differences were found in genotype frequencies for the -16T/C SNP of CALM1 gene between cases and controls (p = 0.581).

Conclusion: Our data implied that the -16TT (rs12885713) CALM1 core promoter genotype is not a risk factor for OA etiology in Greek Caucasians on its own, but associated with the Asporin (ASPN) D14 or D15 risk allele, it could influence KOA susceptibility.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Aged, 80 and over
  • Alleles
  • Calmodulin / genetics*
  • Case-Control Studies
  • Extracellular Matrix Proteins / genetics
  • Female
  • Genetic Predisposition to Disease
  • Greece / ethnology
  • Humans
  • Male
  • Middle Aged
  • Osteoarthritis, Knee / ethnology
  • Osteoarthritis, Knee / genetics*
  • Polymorphism, Single Nucleotide*
  • Promoter Regions, Genetic*
  • Risk Factors
  • White People / genetics*

Substances

  • ASPN protein, human
  • Calmodulin
  • Extracellular Matrix Proteins