PAI-1 donor polymorphism influences long-term kidney graft survival

Nephrol Dial Transplant. 2008 Oct;23(10):3325-32. doi: 10.1093/ndt/gfn241. Epub 2008 May 7.

Abstract

Background: The type 1 plasminogen activator inhibitor (PAI-1) is involved in the development of fibrosis, and its intrarenal expression is increased in interstitial fibrosis and tubular atrophy (IFTA). Moreover, a 4G/5G polymorphism of the PAI-1 gene has been described associating 4G haplotype with higher PAI-1 plasma activity. We investigated the relationship between the donor and recipient PAI-1 polymorphism and kidney graft survival.

Methods: The PAI-1 genotype was determined for both the 304 donors and the 337 corresponding recipients. In recipients, PAI-1 antigen levels were also determined. We compared 4G/4G donors versus donors with other genotypes.

Results: Donor or recipient genotype did not influence the PAI-1 plasma level in recipients. Actuarial kidney graft survival was significantly reduced in the 4G/4G donor group (107 months versus 147.5 months, P = 0.013), while recipient PAI-1 genotype did not show any influence on graft survival. Moreover, graft loss due to IFTA proved significantly higher in the 4G/4G donor group (13% versus 6%, P = 0.03). Multivariate analysis showed that the significant independent variables associated with graft loss were the donor 4G/4G genotype, acute clinical rejection and donor age.

Conclusion: Our study suggests that donor PAI-1 polymorphism influences kidney graft survival and that the donor 4G/4G genotype is an independent risk factor for graft loss. Prospective studies are needed to confirm these results.

MeSH terms

  • Adult
  • Base Sequence
  • DNA Primers / genetics
  • Female
  • Genotype
  • Graft Survival / genetics*
  • Humans
  • Kidney Failure, Chronic / blood
  • Kidney Failure, Chronic / genetics
  • Kidney Failure, Chronic / surgery
  • Kidney Transplantation* / physiology
  • Male
  • Middle Aged
  • Plasminogen Activator Inhibitor 1 / blood
  • Plasminogen Activator Inhibitor 1 / genetics*
  • Polymorphism, Single Nucleotide*
  • Risk Factors
  • Time Factors
  • Tissue Donors
  • Young Adult

Substances

  • DNA Primers
  • Plasminogen Activator Inhibitor 1
  • SERPINE1 protein, human