Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization

R F Suijkerbuijk; A Y van de Veen; J van Echten; C H Buys; B de Jong; J W Oosterhuis; D A Warburton; J J Cassiman; D Schonk; A Geurts van Kessel

Demonstration of the genuine iso-12p character of the standard marker chromosome of testicular germ cell tumors and identification of further chromosome 12 aberrations by competitive in situ hybridization

Am J Hum Genet. 1991 Feb;48(2):269-73.

Authors

R F Suijkerbuijk¹, A Y van de Veen, J van Echten, C H Buys, B de Jong, J W Oosterhuis, D A Warburton, J J Cassiman, D Schonk, A Geurts van Kessel

Affiliation

¹ Department of Human Genetics, University Hospital Nijmegen, The Netherlands.

PMID: 1846721
PMCID: PMC1683009

Abstract

The recently developed competitive in situ hybridization (CISH) strategy was applied to the analysis of chromosome 12 aberrations in testicular germ cell tumors (TGCTs). DNAs from two rodent-human somatic cell hybrids, containing either a normal chromosome 12 or the p arm of chromosome 12 as their unique human material, were used as probes. Our results demonstrate a genuine iso-12p character of the standard marker chromosome in TGCTs. Moreover, variant markers were identified representing translocation products that also involve chromosome 12.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chromosome Aberrations*
Chromosomes, Human, Pair 12*
DNA Probes
DNA, Neoplasm / genetics
Genetic Markers
Humans
Immunohistochemistry
Karyotyping
Male
Neoplasms, Germ Cell and Embryonal / genetics*
Nucleic Acid Hybridization
Testicular Neoplasms / genetics*
Translocation, Genetic
Tumor Cells, Cultured

Substances

DNA Probes
DNA, Neoplasm
Genetic Markers