Effects of glucocorticoids in ventilated piglets with severe pneumonia

Eur Respir J. 2008 Oct;32(4):1037-46. doi: 10.1183/09031936.00009208. Epub 2008 May 28.

Abstract

There is clinical evidence suggesting that glucocorticoids may be useful in severe pneumonia, but the pathogenic mechanisms explaining these beneficial effects are unknown. The aim of the present study was to determine the effects of adding glucocorticoids to antibiotic treatment in an experimental model of severe pneumonia. In total, 15 Lagerwhite-Landrace piglets were ventilated for 96 h. After intubation, a 75 mL solution containing Pseudomonas aeruginosa (10(6) cfu x mL(-1)) was bronchoscopically inoculated. The animals were randomised into three groups 12 h after inoculation: 1) untreated; 2) treated with ciprofloxacin; and 3) treated with ciprofloxacin plus methylprednisolone. Physiological and laboratory parameters were monitored throughout the study. Pro-inflammatory cytokines were measured in serum and bronchoalveolar lavage (BAL). Histopathology of the lungs and cultures from blood, BAL and lungs were performed. At the end of the study, piglets receiving the antibiotic plus glucocorticoids showed: 1) a decrease in the concentration of interleukin-6 in BAL; and 2) a decrease in the global bacterial burden both in BAL and lung tissue. In conclusion, in this experimental model of pneumonia, the association of glucocorticoids with antibiotics attenuates local inflammatory response and decreases bacterial burden in the lung.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Bacterial Agents / pharmacology
  • Anti-Infective Agents / pharmacology
  • Bronchoalveolar Lavage
  • Ciprofloxacin / pharmacology
  • Disease Models, Animal
  • Glucocorticoids / metabolism
  • Glucocorticoids / therapeutic use*
  • Inflammation
  • Lung / drug effects
  • Methylprednisolone / pharmacology
  • Pneumonia / diagnosis
  • Pneumonia / drug therapy*
  • Pseudomonas aeruginosa / metabolism
  • Respiration, Artificial*
  • Swine
  • Time Factors

Substances

  • Anti-Bacterial Agents
  • Anti-Infective Agents
  • Glucocorticoids
  • Ciprofloxacin
  • Methylprednisolone