Abstract
Real-time PCR is a powerful tool for the detection and typing of herpes simplex virus (HSV). HSV types 1 and 2 can be distinguished by using the differences in the melting temperatures (T(m)s) of the hybridization probes. The efficacy of T(m) profiling with low DNA concentrations was evaluated in this study.
MeSH terms
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Cerebrospinal Fluid / virology
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DNA, Viral / chemistry*
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DNA, Viral / genetics*
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Female
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Herpesvirus 2, Human / classification
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Herpesvirus 2, Human / genetics*
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Herpesvirus 2, Human / isolation & purification
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Humans
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Lip / virology
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Male
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Penis / virology
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Tongue / virology
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Transition Temperature*
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Vulva / virology