Atrial gap junctions, NF-kappaB and fibrosis in patients undergoing coronary artery bypass surgery: the relationship with postoperative atrial fibrillation

Cardiology. 2009;112(2):81-8. doi: 10.1159/000141012. Epub 2008 Jun 26.

Abstract

Objectives: We examined the role of atrial gap junctions, NF-kappaB and fibrosis in the occurrence of postoperative atrial fibrillation (AF) in patients undergoing coronary artery bypass graft (CABG) surgery.

Methods: Forty-five patients with sinus rhythm were randomly assigned to the beating heart (n = 22) or cardioplegic cardiac arrest (n = 23) technique for surgery. Of them, 14 patients experienced post-CABG AF. Atrial samples taken before and after CABG surgery were analyzed.

Results: During surgery, Cx43 and Cx40 proteins were significantly reduced (both p < 0.05) in the arrested heart group, but only mildly decreased in the beating heart group. However, the change of either connexin was not associated with AF. In contrast, patients with AF had a higher baseline expression of NF-kappaB and more fibrosis compared to those without AF (both p < 0.05).

Conclusions: CABG surgery with the beating heart technique attenuated the reduction of atrial Cx43 and Cx40 compared to the cardioplegic cardiac arrest technique. Atrial inflammation and fibrosis status before surgery, but not the changes of connexins during surgery, were associated with the occurrence of post-CABG AF.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aged
  • Atrial Fibrillation / metabolism
  • Atrial Fibrillation / pathology*
  • Connexin 43 / metabolism
  • Connexins / metabolism
  • Coronary Artery Bypass, Off-Pump*
  • Female
  • Fibrosis
  • Gap Junction alpha-5 Protein
  • Gap Junctions / metabolism
  • Gap Junctions / pathology*
  • Heart Arrest, Induced*
  • Heart Atria / metabolism
  • Heart Atria / pathology
  • Heart Atria / surgery
  • Humans
  • Male
  • Middle Aged
  • NF-kappa B / metabolism*
  • Postoperative Complications / metabolism
  • Postoperative Complications / pathology*

Substances

  • Connexin 43
  • Connexins
  • GJA1 protein, human
  • NF-kappa B