Antifungal resistance of Candida glabrata vaginal isolates and development of a quantitative reverse transcription-PCR-based azole susceptibility assay

Antimicrob Agents Chemother. 2008 Sep;52(9):3424-6. doi: 10.1128/AAC.00462-08. Epub 2008 Jun 30.

Abstract

A multiplex quantitative reverse transcription-PCR assay was developed to detect azole resistance in Candida glabrata, an important opportunistic pathogen that develops resistance rapidly. Resistance was defined as a >or=3-fold increase in CDR1 expression by this assay, which proved to be 100% sensitive and 95% specific in comparison to the gold standard broth microdilution assay.

Publication types

  • Evaluation Study

MeSH terms

  • Antifungal Agents / pharmacology*
  • Azoles / pharmacology*
  • Candida glabrata / drug effects*
  • Candida glabrata / isolation & purification
  • Candidiasis, Vulvovaginal / microbiology
  • Drug Resistance, Fungal*
  • Female
  • Fluconazole
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism
  • Gene Expression Regulation, Fungal
  • Humans
  • Membrane Transport Proteins / genetics
  • Membrane Transport Proteins / metabolism
  • Microbial Sensitivity Tests / methods
  • Reverse Transcriptase Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Up-Regulation
  • Vagina / microbiology*

Substances

  • Antifungal Agents
  • Azoles
  • CDR1 protein, Candida albicans
  • Fungal Proteins
  • Membrane Transport Proteins
  • Fluconazole