A C. elegans homolog of huntingtin-associated protein 1 is expressed in chemosensory neurons and in a number of other somatic cell types

J Mol Neurosci. 2009 Jan;37(1):37-49. doi: 10.1007/s12031-008-9109-z. Epub 2008 Jul 1.

Abstract

Huntingtin-associated protein 1 (HAP1) is a binding partner for huntingtin, the protein responsible for Huntington's disease. In mammals, HAP1 is mostly found in brain where it is expressed in neurons. Although several functions have been proposed for HAP1, its role has not yet been clearly established. In this paper, we report on the identification of a HAP1 Caenorhabditis elegans homolog called T27A3.1. T27A3.1 shows conservation with rat and human HAP1, as well as with Milton, a Drosophila HAP1 homolog. To determine the cellular expression of T27A3.1 (multiple isoforms; a-e), we generated several transgenic worm lines expressing a fluorescent reporter protein [green fluorescent protein (GFP) and DsRed2] under the control of the promoter for T27A3.1. We have found that T27A3.1 is expressed in many cell types including a subset of chemosensory neurons in the head and tail. These include the amphid chemosensory neurons ASKL and R, ASIL and R, ADFL and ASEL, the phasmid neurons PHBL and R, and the CAN neurons that are required for worm survival.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Base Sequence
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans Proteins / genetics*
  • Chemoreceptor Cells / physiology*
  • Cloning, Molecular
  • Green Fluorescent Proteins / genetics
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Promoter Regions, Genetic / genetics
  • RNA Interference

Substances

  • Caenorhabditis elegans Proteins
  • Nerve Tissue Proteins
  • T27A3.1 protein, C elegans
  • Green Fluorescent Proteins