Abstract
The ability of the low density lipoprotein receptor-related protein (LRP) to form homo-dimers was studied in mouse neuroblastoma and human neuroglioma cells as well as in primary cortical cultures from adult mouse brain. Homo-dimerization of LRP light chain (LC) was shown by several methods including co-immunoprecipitation, fluorescence lifetime imaging microscopy, and bimolecular fluorescence complementation assay. The requirement of intact NPXY motifs of LRP LC for homo-dimerization was ruled out by co-immunoprecipitation assay.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Amino Acid Motifs
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Animals
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Cells, Cultured
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Cerebral Cortex / cytology
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Embryo, Mammalian
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Endocytosis
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Fluorescence Resonance Energy Transfer / methods
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Humans
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Low Density Lipoprotein Receptor-Related Protein-1 / genetics
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Low Density Lipoprotein Receptor-Related Protein-1 / metabolism*
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Mice
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Neurons / metabolism*
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Protein Processing, Post-Translational
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Sequence Homology, Amino Acid*
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Transfection / methods
Substances
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Low Density Lipoprotein Receptor-Related Protein-1
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Green Fluorescent Proteins