The Genome Sequencer FLX System--longer reads, more applications, straight forward bioinformatics and more complete data sets

J Biotechnol. 2008 Aug 31;136(1-2):3-10. doi: 10.1016/j.jbiotec.2008.03.021. Epub 2008 Jun 21.

Abstract

The Genome Sequencer FLX System (GS FLX), powered by 454 Sequencing, is a next-generation DNA sequencing technology featuring a unique mix of long reads, exceptional accuracy, and ultra-high throughput. It has been proven to be the most versatile of all currently available next-generation sequencing technologies, supporting many high-profile studies in over seven applications categories. GS FLX users have pursued innovative research in de novo sequencing, re-sequencing of whole genomes and target DNA regions, metagenomics, and RNA analysis. 454 Sequencing is a powerful tool for human genetics research, having recently re-sequenced the genome of an individual human, currently re-sequencing the complete human exome and targeted genomic regions using the NimbleGen sequence capture process, and detected low-frequency somatic mutations linked to cancer.

Publication types

  • Review

MeSH terms

  • Algorithms*
  • Base Sequence
  • Chromosome Mapping / methods*
  • Computational Biology / methods*
  • Database Management Systems
  • Databases, Genetic*
  • Molecular Sequence Data
  • Sequence Analysis, DNA / methods*
  • Software Design
  • Software*
  • User-Computer Interface*