Trypanosoma cruzi in non-human primates with a history of stillbirths: a retrospective study (Papio hamadryas spp.) and case report (Macaca fascicularis)

J Med Primatol. 2008 Dec;37(6):318-28. doi: 10.1111/j.1600-0684.2008.00302.x. Epub 2008 Jul 30.

Abstract

Background: Congenital transmission of Trypanosoma cruzi has been described in humans and experimental work has been conducted with mice, but not with non-human primates (NHPs).

Methods: We conducted a retrospective study of female baboons (Papio hamadryas spp.) naturally seropositive or seronegative for T. cruzi with history of fetal loss, and we report a stillbirth in a cynomolgus macaque (Macaca fascicularis) with placental T. cruzi amastigotes.

Results: There were no differences in menstrual cycle parameters and the number of fetal losses between seropositive and seronegative baboons with history of fetal loss. The amount of parasite DNA detected using quantitative polymerase chain reaction (Q-PCR) in M. fascicularis placenta was within the range detected in infected baboon tissues.

Conclusions: There is no evidence that chronic maternal T. cruzi infection causes fetal loss in baboons. Q-PCR is a useful diagnostic tool to study archived NHP placentas.

Publication types

  • Case Reports
  • Research Support, N.I.H., Extramural

MeSH terms

  • Animals
  • Ape Diseases / parasitology*
  • Chagas Disease / parasitology
  • Chagas Disease / veterinary*
  • DNA, Protozoan / chemistry
  • DNA, Protozoan / genetics
  • Fatal Outcome
  • Female
  • Immunohistochemistry
  • Macaca fascicularis
  • Male
  • Papio*
  • Placenta / parasitology
  • Polymerase Chain Reaction / veterinary
  • Pregnancy
  • Retrospective Studies
  • Stillbirth / veterinary*
  • Trypanosoma cruzi / growth & development*

Substances

  • DNA, Protozoan