Abstract
Estrogens are a class of steroid hormones that interact with two related but distinct nuclear receptors, estrogen receptor (ER) alpha and beta. To identify potential ER biomarkers, we profiled the rat plasma glycoproteome after treatment with vehicle or 17beta-estradiol (E2) or an ERalpha-selective agonist PPT by differential mass spectrometry. Our comparative proteomic experiment identifies novel E2- and PPT-responsive proteins, such as serine protease inhibitor family members.
MeSH terms
-
Amino Acid Sequence
-
Animals
-
Blood Proteins / chemistry
-
Blood Proteins / genetics
-
Blood Proteins / metabolism*
-
Estradiol / metabolism*
-
Female
-
Glycoproteins / chemistry
-
Glycoproteins / genetics
-
Glycoproteins / metabolism
-
Male
-
Mass Spectrometry / methods*
-
Molecular Sequence Data
-
Organ Size
-
Peptides / chemistry
-
Peptides / genetics
-
Peptides / metabolism
-
Phenols / metabolism*
-
Plasma / chemistry*
-
Pyrazoles / metabolism*
-
Rats
-
Rats, Sprague-Dawley
-
Receptors, Estrogen / agonists
-
Receptors, Estrogen / metabolism
-
Selective Estrogen Receptor Modulators / metabolism*
-
Uterus / anatomy & histology
Substances
-
Blood Proteins
-
Glycoproteins
-
Peptides
-
Phenols
-
Pyrazoles
-
Receptors, Estrogen
-
Selective Estrogen Receptor Modulators
-
4,4',4''-(4-propyl-((1)H)-pyrazole-1,3,5-triyl) tris-phenol
-
Estradiol