A prospective evaluation of the effect of sample collection site on intraoperative parathormone monitoring during parathyroidectomy

Surgery. 2008 Oct;144(4):504-9; discussion 509-10. doi: 10.1016/j.surg.2008.07.004. Epub 2008 Aug 29.

Abstract

Background: Sample collection site may affect the dynamics of intraoperative parathyroid hormone monitoring (IPM) and influence surgical decisions.

Methods: We prospectively studied 45 patients undergoing parathyroidectomy for primary hyperparathyroidism. The IPM cure criterion was a decrease in peripheral vein (PV) parathyroid hormone (PTH) of >50% at 10 minutes after gland excision. PTH samples were collected simultaneously from PV and central vein (CV) and compared for PTH decay, the incidence of >50% PTH decay, and the incidence of normal PTH values after gland excision.

Results: Mean PTH levels were significantly higher from the CV before and after gland excision. Mean PTH decay 10 minutes after gland excision was 89% PV versus 88% CV, resulting in mean PTH levels of 27 +/- 23 and 39 +/- 35 pg/mL, respectively (P < .0001). At 5 minutes, >50% decay in PTH was present in 98% PV versus 88% CV samples. By 10 minutes, the incidence of >50% PTH decay was equivalent (98%). This yielded normal range PTH levels from the PV versus CV in 90% versus 76% of patients at 5 minutes, 96% versus 89% at 10 minutes, and 95% versus 81% at 20 minutes. Of 45 patients, 44 (98%) are normocalcemic at a mean follow-up of 6.3 months. IPM predicted the single operative failure.

Conclusions: CV sampling produces significantly higher PTH levels. Surgeons sampling from a PV may observe a >50% decrease in PTH and normal range PTH values starting 5 minutes after gland excision. Surgeons who sample from the CV and require normalization of PTH levels may have to wait longer and/or continue potentially unnecessary neck exploration.

MeSH terms

  • Adult
  • Aged
  • Blood Specimen Collection / methods*
  • Evaluation Studies as Topic
  • Female
  • Follow-Up Studies
  • Humans
  • Hyperparathyroidism, Primary / diagnosis
  • Hyperparathyroidism, Primary / surgery*
  • Male
  • Middle Aged
  • Minimally Invasive Surgical Procedures / methods
  • Monitoring, Intraoperative / methods*
  • Parathyroid Hormone / analysis*
  • Parathyroidectomy / methods*
  • Probability
  • Prospective Studies
  • Sensitivity and Specificity
  • Treatment Outcome

Substances

  • Parathyroid Hormone