Using post-genomic technologies, it is now possible to understand the molecular basis of complex developmental processes. In the case of seed germination, recent transcriptome- and proteome-wide studies led to new insights concerning the building up of the germination potential during seed maturation on the mother plant, the reversible character of the first phases of the germination process enabling the imbibed embryo to recapitulate the late maturation program for mounting defense response when confronted to environmental fluctuations, the timing of expression of genes playing a role in controlling radicle emergence, the role of plant hormones as abscisic acid and gibberellins in seed germination, and finally the global changes in proteome activity induced by redox regulation occurring in seed development and germination. In this way, post-genomic technologies help facilitating the advent of a systems approach to uncover novel features of seed quality, which can lead to potential applications, for example in selection programs.