Distinct MRI pattern in lesional and perilesional area after traumatic brain injury in rat--11 months follow-up

Exp Neurol. 2009 Jan;215(1):29-40. doi: 10.1016/j.expneurol.2008.09.009. Epub 2008 Sep 27.

Abstract

To understand the dynamics of progressive brain damage after lateral fluid-percussion induced traumatic brain injury (TBI) in rat, which is the most widely used animal model of closed head TBI in humans, MRI follow-up of 11 months was performed. The evolution of tissue damage was quantified using MRI contrast parameters T(2), T(1rho), diffusion (D(av)), and tissue atrophy in the focal cortical lesion and adjacent areas: the perifocal and contralateral cortex, and the ipsilateral and contralateral hippocampus. In the primary cortical lesion area, which undergoes remarkable irreversible pathologic changes, MRI alterations start at 3 h post-injury and continue to progress for up to 6 months. In more mildly affected perifocal and hippocampal regions, the robust alterations in T(2), T(1rho), and D(av) at 3 h to 3 d post-injury normalize within the next 9-23 d, and thereafter, progressively increase for several weeks. The severity of damage in the perifocal and hippocampal areas 23 d post-injury appeared independent of the focal lesion volume. Magnetic resonance spectroscopy (MRS) performed at 5 and 10 months post-injury detected metabolic alterations in the ipsilateral hippocampus, suggesting ongoing neurodegeneration and inflammation. Our data show that TBI induced by lateral fluid-percussion injury triggers long-lasting alterations with region-dependent temporal profiles. Importantly, the temporal pattern in MRI parameters during the first 23 d post-injury can indicate the regions that will develop secondary damage. This information is valuable for targeting and timing interventions in studies aiming at alleviating or reversing the molecular and/or cellular cascades causing the delayed injury.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aspartic Acid / analogs & derivatives
  • Aspartic Acid / metabolism
  • Brain / metabolism*
  • Brain / pathology*
  • Brain Chemistry / physiology
  • Brain Injuries / pathology*
  • Creatine / metabolism
  • Diffusion Magnetic Resonance Imaging / methods*
  • Dipeptides / metabolism
  • Disease Models, Animal
  • Follow-Up Studies
  • Image Processing, Computer-Assisted / methods
  • Magnetic Resonance Imaging / methods*
  • Magnetic Resonance Spectroscopy / methods*
  • Male
  • Rats
  • Rats, Sprague-Dawley
  • Severity of Illness Index
  • Statistics, Nonparametric
  • Time Factors

Substances

  • Dipeptides
  • isospaglumic acid
  • Aspartic Acid
  • N-acetylaspartate
  • Creatine