Assembly of empty capsids by using baculovirus recombinants expressing human parvovirus B19 structural proteins

J Virol. 1991 May;65(5):2702-6. doi: 10.1128/JVI.65.5.2702-2706.1991.

Abstract

Empty parvovirus B19 capsids were isolated from insect cells infected with a recombinant baculovirus expressing parvovirus B19 VP2 alone and also with a double-recombinant baculovirus expressing both VP1 and VP2. That VP2 alone can assemble to form capsids is a phenomenon not previously observed in parvoviruses. The stoichiometry of the capsids containing both VP1 and VP2 was similar to that previously observed in parvovirus B19-infected cells. The capsids were similar to native capsids in size and appearance, and their antigenicity was demonstrated by immunoprecipitation and enzyme-linked immunosorbent assay with B19-specific antibodies.

MeSH terms

  • Animals
  • Baculoviridae / genetics*
  • Capsid / biosynthesis*
  • Capsid / genetics
  • Capsid Proteins
  • Cell Line
  • DNA, Recombinant
  • Drosophila melanogaster
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Moths
  • Parvoviridae / genetics*
  • Parvoviridae / metabolism
  • Precipitin Tests
  • Recombinant Proteins / biosynthesis
  • Restriction Mapping
  • Viral Structural Proteins / biosynthesis*
  • Viral Structural Proteins / genetics

Substances

  • Capsid Proteins
  • DNA, Recombinant
  • Recombinant Proteins
  • Viral Structural Proteins