Increase of auto anti-phosphatidylinositol antibodies in plasma of female rats during the appearance of DMBA-induced malignant mammary tumors

Cancer Lett. 1991 Apr;57(1):15-9. doi: 10.1016/0304-3835(91)90057-o.

Abstract

Using a lipid-adapted ELISA, antiphosphatidylinositol (PtdIns) antibodies (Ab) have been found in sera of cancer patients. With the same procedure, we have checked their possible raising in plasma of female rats during the appearance of 7,12-dimethylbenz[a]anthracene (DMBA)-induced mammary tumors. Twenty days after DMBA administration, the mean anti-PtdIns Ab level was already higher than that of control rats (oil). Immunochemical analysis of the anti-PtdIns Ab site showed a rather high Ab avidity (8 x 10(-9) M, at half-displacement) and high specificity for glyceryl phosphate inositol residues. Other phospholipids (PL) were not recognized by the anti-PtdIns Ab induced by malignant cell transformation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 9,10-Dimethyl-1,2-benzanthracene
  • Animals
  • Antibody Affinity
  • Antibody Specificity
  • Autoantibodies / analysis*
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Mammary Neoplasms, Experimental / chemically induced
  • Mammary Neoplasms, Experimental / immunology*
  • Phosphatidylinositols / immunology*
  • Rats
  • Rats, Inbred Strains
  • Reference Values
  • Time Factors

Substances

  • Autoantibodies
  • Phosphatidylinositols
  • 9,10-Dimethyl-1,2-benzanthracene