Abstract
S100B is an astrocyte-derived cytokine implicated in the IL-1beta-triggered cytokine cycle in Alzheimer's disease. However, the secretion of S100B following stimulation by IL-1beta has not been directly demonstrated. We investigated S100B secretion in cortical primary astrocyte cultures, C6 glioma cells and acute hippocampal slices exposed to IL-1beta. S100B secretion was induced by IL-1beta in all preparations, involving MAPK pathway and, apparently, NF-small ka, CyrillicB signaling. Astrocytes and C6 cells exhibited different sensitivities to IL-1beta. These results suggest that IL-1beta-induced S100B secretion is a component of the neuroinflammatory response, which would support the involvement of S100B in the genesis of neurodegenerative diseases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Animals
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Animals, Newborn
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Cells, Cultured
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Cerebral Cortex / cytology
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Dose-Response Relationship, Drug
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Enzyme Inhibitors / pharmacology
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Hippocampus / drug effects*
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In Vitro Techniques
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Indoles
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Interleukin-1beta / pharmacology*
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L-Lactate Dehydrogenase / metabolism
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Mitogen-Activated Protein Kinase Kinases / metabolism*
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Nerve Growth Factors / metabolism*
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Neuroglia / drug effects*
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Nitric Oxide / metabolism
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Rats
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Rats, Wistar
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins / metabolism*
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Signal Transduction / drug effects
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Signal Transduction / physiology*
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Subcellular Fractions / drug effects
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Time Factors
Substances
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Enzyme Inhibitors
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Indoles
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Interleukin-1beta
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Nerve Growth Factors
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S100 Calcium Binding Protein beta Subunit
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S100 Proteins
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S100b protein, rat
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Nitric Oxide
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DAPI
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L-Lactate Dehydrogenase
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Mitogen-Activated Protein Kinase Kinases