Severe pneumonia with Leptotrichia sp. detected predominantly in bronchoalveolar lavage fluid by use of 16S rRNA gene sequencing analysis

Toshinori Kawanami; Kazumasa Fukuda; Kazuhiro Yatera; Takashi Kido; Chiharu Yoshii; Hatsumi Taniguchi; Masamitsu Kido

doi:10.1128/JCM.01429-08

Severe pneumonia with Leptotrichia sp. detected predominantly in bronchoalveolar lavage fluid by use of 16S rRNA gene sequencing analysis

J Clin Microbiol. 2009 Feb;47(2):496-8. doi: 10.1128/JCM.01429-08. Epub 2008 Dec 3.

Authors

Toshinori Kawanami¹, Kazumasa Fukuda, Kazuhiro Yatera, Takashi Kido, Chiharu Yoshii, Hatsumi Taniguchi, Masamitsu Kido

Affiliation

¹ Department of Respiratory Disease, University of Occupational and Environmental Health, Fukuoka, Japan.

Abstract

We present the first case of severe pneumonia possibly caused by Leptotrichia species with oral bacteria. This was found in a healthy but elderly subject whose bronchoalveolar lavage fluid was analyzed by 16S rRNA gene sequencing analysis. The combination of this method and microscopic observation provided useful information for diagnosis and treatment.

Publication types

Case Reports

MeSH terms

Aged
Bronchoalveolar Lavage Fluid / microbiology*
DNA, Bacterial / chemistry
DNA, Bacterial / genetics
DNA, Ribosomal / chemistry
DNA, Ribosomal / genetics
Fusobacteriaceae Infections / diagnosis*
Fusobacteriaceae Infections / microbiology
Humans
Leptotrichia / genetics
Leptotrichia / isolation & purification*
Male
Pneumonia, Bacterial / diagnosis*
Pneumonia, Bacterial / microbiology
RNA, Ribosomal, 16S / genetics
Radiography, Thoracic
Sequence Analysis, DNA

Substances

DNA, Bacterial
DNA, Ribosomal
RNA, Ribosomal, 16S