Optical resolution below lambda/4 using synthetic aperture microscopy and evanescent-wave illumination

Opt Express. 2008 Dec 8;16(25):20477-83. doi: 10.1364/oe.16.020477.

Abstract

Evanescent-wave illumination is applied to synthetic-aperture microscopy on a transparent solid substrate to extend the resolution limit to lambda/2(n+1) (where n is the substrate refractive index) independent of the lens NA. Using a 633 nm source and a 0.4 NA lens, a resolution to 150 nm (lambda/4.2) is demonstrated on a glass (n = 1.5) substrate. Further extension to approximately 74-nm resolution (lambda/8.6) is projected with a higher index substrate (n = 3.3).

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Computer Simulation
  • Computer-Aided Design*
  • Equipment Design
  • Equipment Failure Analysis
  • Image Enhancement / instrumentation*
  • Lenses*
  • Light
  • Lighting / instrumentation*
  • Microscopy / instrumentation*
  • Models, Theoretical*
  • Scattering, Radiation