Localization of C. trachomatis Inc proteins in expression of their genes in HeLa cell culture

Bull Exp Biol Med. 2008 Aug;146(2):237-42. doi: 10.1007/s10517-008-0254-8.
[Article in English, Russian]

Abstract

Co-localization of Chlamydia trachomatis incorporation membrane proteins with cell organelles was studied in HeLa cell culture after transfection by expressing vectors carrying incA, incB, incC, incD, incE, incF, incG genes, respectively, fused with the marker green fluorescent protein (EGFP) gene. The prokaryotic proteins were co-located with compartments of the secretory pathway of the eukaryotic cell in the course of biogenesis.

MeSH terms

  • Bacterial Proteins / metabolism*
  • Chlamydia trachomatis / genetics*
  • Gene Expression
  • Genes, Bacterial
  • Green Fluorescent Proteins
  • HeLa Cells
  • Humans
  • Membrane Proteins / metabolism*
  • Microscopy, Confocal
  • Microscopy, Fluorescence
  • Organelles / metabolism*
  • Recombinant Fusion Proteins / metabolism
  • Transfection

Substances

  • Bacterial Proteins
  • IncA protein, Chlamydia trachomatis
  • Membrane Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins