Local electrical stimulation of cultured embryonic cardiomyocytes with sub-micrometer nail structures

Annu Int Conf IEEE Eng Med Biol Soc. 2008:2008:4816-9. doi: 10.1109/IEMBS.2008.4650291.

Abstract

In this paper, we demonstrate the feasibility of selective extracellular electrical stimulation at the (sub)cellular level in dissociated cultured cells. Using a CMOS-compatible process, we have fabricated an electrode array with sub-micrometer nail probes. Due to their particular configuration, the nails are strongly engulfed by the cellular membrane. By measuring the calcium signals, we found that electrical stimulation via the micronails activates the cell locally, in a dose-dependent manner, with very low applied currents. The results suggest the applicability of the device in pharmacological or signal propagation studies.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Action Potentials / physiology*
  • Animals
  • Cells, Cultured
  • Electric Stimulation / instrumentation*
  • Electric Stimulation / methods
  • Embryonic Stem Cells / cytology
  • Embryonic Stem Cells / physiology*
  • Equipment Design
  • Equipment Failure Analysis
  • Microelectrodes*
  • Myocytes, Cardiac / cytology
  • Myocytes, Cardiac / physiology*
  • Rats
  • Rats, Wistar
  • Signal Processing, Computer-Assisted / instrumentation*