RNAither, an automated pipeline for the statistical analysis of high-throughput RNAi screens

Bioinformatics. 2009 Mar 1;25(5):678-9. doi: 10.1093/bioinformatics/btp014. Epub 2009 Jan 25.

Abstract

Summary: We present RNAither, a package for the free statistical environment R which performs an analysis of high-throughput RNA interference (RNAi) knock-down experiments, generating lists of relevant genes and pathways out of raw experimental data. The library provides a quality assessment of the signal intensities, as well as a broad range of options for data normalization, different statistical tests for the identification of significant siRNAs, and a significance analysis of the biological processes involving corresponding genes. The results of the analysis are presented as a set of HTML pages. Additionally, all values and plots are available as either text files or pdf and png files.

Availability: http://bioconductor.org/

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Computational Biology / methods
  • Data Interpretation, Statistical
  • Gene Expression Profiling / methods
  • RNA Interference*
  • RNA, Small Interfering / chemistry
  • Software*

Substances

  • RNA, Small Interfering