Rrp2, a sigma54-dependent transcriptional activator of Borrelia burgdorferi, activates rpoS in an enhancer-independent manner

J Bacteriol. 2009 Apr;191(8):2902-5. doi: 10.1128/JB.01721-08. Epub 2009 Feb 6.

Abstract

Rrp2 is the sole sigma(54)-dependent transcriptional activator present in the Borrelia burgdorferi genome. We showed that recombinant Rrp2 binds to DNA in a sequence-nonspecific manner. During infection, Rrp2 activates sigma(54)-dependent rpoS expression without an apparent upstream enhancer element commonly associated with other sigma(54)-dependent transcriptional activators.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / biosynthesis*
  • Bacterial Proteins / genetics
  • Borrelia burgdorferi / physiology*
  • DNA, Bacterial / metabolism
  • Electrophoretic Mobility Shift Assay
  • Gene Expression Regulation, Bacterial*
  • Promoter Regions, Genetic
  • Protein Binding
  • RNA Polymerase Sigma 54 / metabolism
  • Sigma Factor / biosynthesis*
  • Sigma Factor / genetics
  • Trans-Activators / metabolism*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Sigma Factor
  • Trans-Activators
  • sigma factor KatF protein, Bacteria
  • RNA Polymerase Sigma 54