Screening of SMG7-binding peptides by combination of phage display and docking simulation analysis

Protein Pept Lett. 2009;16(3):301-5. doi: 10.2174/092986609787601741.

Abstract

We screened SMG7-binding peptides with phage display and docking simulation analysis. Although a consensus motif was absent in the phage display-derived candidates, we succeeded to find a peptide CDDRPPKSC, which can bind specifically to SMG7. We conclude that docking simulation helps to find high-affinity peptides efficiently, even if the phage display-screened candidates lack a consensus region.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carrier Proteins / metabolism*
  • Computer Simulation
  • Humans
  • Peptide Library
  • Peptides / metabolism*
  • Protein Binding

Substances

  • Carrier Proteins
  • Peptide Library
  • Peptides
  • SMG7 protein, human