Spinal cord hydrolysate ameliorate immunological reaction in experimental allergic encephalomyelitis

Acta Neurobiol Exp (Wars). 2009;69(1):73-8. doi: 10.55782/ane-2009-1731.

Abstract

The aim of this study was to use the hydrolysate of pig spinal cord proteins to induce oral tolerance in the animal model of sclerosis multiplex - experimental allergic encephalomyelitis. The female Lewis rats were fed with hydrolysate of pig spinal cord proteins in two doses for one week before immunization, which was induced by injection of guinea pig spinal cord homogenate. At the peak of clinical symptoms (the 13th day post immunization) the rats were sacrificed and the spleen removed. Splenocytes were suspended in a culture medium and placed in microculture plates. The cells were stimulated with homogenate. The cells were cultured for seven days. Proliferation of splenocytes was estimated by means of methyl-3H thymidine incorporation. In supernatants of cultures of splenocytes the level of cytokines INF-gamma, IL-10, IL-4, and TGF-gamma was measured. It was demonstrated that homogenate-induced splenocytes of hydrolysate-fed rats gave rise to low proliferation as compared to the controls used. The IFN-gamma was inhibited in hydrolysate-fed animals. The hydrolysate of pig spinal cord proteins has a modulatory effect on the immune reaction, particularly on the orally-induced antigen-specific modulation of autoimmune response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Proliferation / drug effects
  • Cells, Cultured
  • Disease Models, Animal
  • Encephalomyelitis, Autoimmune, Experimental / etiology
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / prevention & control
  • Enzyme-Linked Immunosorbent Assay / methods
  • Female
  • Guinea Pigs
  • Interferon-gamma / metabolism*
  • Interleukin-10 / metabolism*
  • Lymphocytes / drug effects
  • Lymphocytes / immunology
  • Protein Hydrolysates / immunology*
  • Protein Hydrolysates / pharmacology
  • Rats
  • Rats, Inbred Lew
  • Severity of Illness Index
  • Spinal Cord / cytology
  • Spinal Cord / metabolism*
  • Spleen / cytology
  • Time Factors

Substances

  • Protein Hydrolysates
  • Interleukin-10
  • Interferon-gamma