Separation and molecular characterization of mycolic acid from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) and BCG substrains by normal-phase high performance liquid chromatography and liquid chromatography/mass spectrometry

Yuko Uenishi; Takemasa Takii; Ikuya Yano; Makoto Sunagawa

doi:10.1016/j.mimet.2009.03.006

Separation and molecular characterization of mycolic acid from the cell wall skeleton of Mycobacterium bovis BCG Tokyo 172 (SMP-105) and BCG substrains by normal-phase high performance liquid chromatography and liquid chromatography/mass spectrometry

J Microbiol Methods. 2009 Jun;77(3):320-2. doi: 10.1016/j.mimet.2009.03.006. Epub 2009 Mar 27.

Authors

Yuko Uenishi¹, Takemasa Takii, Ikuya Yano, Makoto Sunagawa

Affiliation

¹ Technology Research & Development Division, Dainippon Sumitomo Pharma Co., Ltd., 3-1-98, Kasugade-naka, Konohana-ku, Osaka 554-0022, Japan. yuko-uenishi@ds-pharma.co.jp

PMID: 19328829
DOI: 10.1016/j.mimet.2009.03.006

Abstract

Since mycolic acids, the most characteristic major lipid component in mycobacterial cell envelopes, play pivotal roles in the cell surface-based host immune responses, normal-phase HPLC has been developed to quantify and identify mycolic acids of the cell wall skeleton from Mycobacterium bovis BCG Tokyo 172 (SMP-105).

Publication types

Evaluation Study

MeSH terms

Cell Wall Skeleton / chemistry*
Chromatography, High Pressure Liquid / methods*
Chromatography, Liquid / methods*
Mass Spectrometry / methods*
Mycobacterium bovis / chemistry*
Mycolic Acids / chemistry*

Substances

Cell Wall Skeleton
Mycolic Acids