[Implanting the conducting electrode in rat and investigating its effect on the rat's penicillin-induced seizure]

Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2009 Feb;26(1):63-6.
[Article in Chinese]

Abstract

To investigate the effects of conducting electrodes implanted into the penicillin-induced epilepsy foci in rats on seizures, 30 male SD rats were randomly divided into experimental group (EG) and control group (CG). Afer penicillin(PNC) administration, metal electrode was implanted into the epilepsy focus in EG. Behavior evaluations were given after PNC injection in both groups and electroencephalogram recordings were taken respectively. Immunohistochemical methods were used to test the expressions of P38 and Calcium channel a 1A subunit (CACNA1A) at the serial time-point. We found that EG showed reduction of epileptic discharge frequency than did CG at time-point 3 h and 6 h, and that P38 expressions of EG were lower than those of CG in the injected side, and the expressions of Calcium channel a 1A subunit (CACNA1A) were downregulated in EG at time-point 6 h and 24 h. This evidence suggests that the metal electrode implanted into the penicillin-induced epilepsy focus may reduce the epileptic discharges and downregulate the expressions of P38 and CACNA1A. This new idea might be used in antiepileptic field in future.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium Channels / metabolism
  • Down-Regulation
  • Electrodes, Implanted*
  • Electroencephalography*
  • Epilepsy / chemically induced
  • Epilepsy / therapy*
  • Male
  • Penicillins
  • Rats
  • Rats, Sprague-Dawley
  • p38 Mitogen-Activated Protein Kinases / metabolism

Substances

  • Cacna1a protein, rat
  • Calcium Channels
  • Penicillins
  • p38 Mitogen-Activated Protein Kinases