Interferon-alpha treatment of Daudi cells down-regulates the octamer binding transcription/DNA replication factors Oct-1 and Oct-2

J Biol Chem. 1991 Nov 5;266(31):20888-92.

Abstract

Treatment of Daudi cells with alpha-interferon (alpha-IFN) results in a considerable decrease in the levels of the octamer-binding DNA replication/transcription factors Oct-1 and Oct-2 and specifically inhibits gene expression by octamer-containing promoters. The inhibitory effect on octamer-binding proteins also occurs after culturing cells with phorbol 12-myristate 13-acetate but it does not occur following alpha-IFN treatment of an alpha-IFN-resistant variant of the Daudi cell line or of HeLa cells. We discuss the potential role of the decreased levels of octamer-binding proteins in the inhibition of cell proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Cell Cycle
  • DNA / biosynthesis
  • DNA-Binding Proteins / genetics
  • DNA-Binding Proteins / metabolism*
  • Gene Expression / drug effects
  • Host Cell Factor C1
  • Humans
  • In Vitro Techniques
  • Interferon Type I / pharmacology*
  • Molecular Sequence Data
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Oligonucleotides / chemistry
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Recombinant Proteins
  • Transcription Factors / genetics
  • Transcription Factors / metabolism*
  • Transcription, Genetic
  • Tumor Cells, Cultured

Substances

  • DNA-Binding Proteins
  • HCFC1 protein, human
  • Host Cell Factor C1
  • Interferon Type I
  • Octamer Transcription Factor-1
  • Octamer Transcription Factor-2
  • Oligonucleotides
  • POU2F1 protein, human
  • POU2F2 protein, human
  • RNA, Messenger
  • Recombinant Proteins
  • Transcription Factors
  • DNA