Abstract
Assays that use rhesus macaque whole blood and an antigen capture enzyme-linked immunosorbent assay for the simian immunodeficiency virus (SIV) p27 core protein were developed for the isolation of SIV from the blood of infected animals, the titration of infectivity of SIV inocula, and the quantitation of virus neutralizing antibodies in serum. These assays required small amounts of whole blood, were adaptable to a microtiter format, and used substrates mainly of rhesus macaque origin.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Antibodies, Viral
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Antigens, Viral / blood
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Enzyme-Linked Immunosorbent Assay / methods*
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Evaluation Studies as Topic
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Gene Products, gag / immunology
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Macaca mulatta
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Neutralization Tests
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Simian Acquired Immunodeficiency Syndrome / blood
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Simian Acquired Immunodeficiency Syndrome / microbiology*
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Simian Immunodeficiency Virus / immunology
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Simian Immunodeficiency Virus / isolation & purification*
Substances
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Antibodies, Viral
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Antigens, Viral
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Gag protein p27, Simian immunodeficiency virus
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Gene Products, gag