Molecular analyses of the rice glutamate dehydrogenase gene family and their response to nitrogen and phosphorous deprivation

Plant Cell Rep. 2009 Jul;28(7):1115-26. doi: 10.1007/s00299-009-0709-z. Epub 2009 May 9.

Abstract

Glutamate dehydrogenases (GDH, EC 1.4.1.2 approximately 4) are ubiquitous enzymes encoded by GDH genes. So far, at least two GDH members have been characterized in plants, but most members of this family in rice remains to be characterized. Here, we show that four putative GDH genes (OsGDH1-4) are present in the rice genome. The GDH sequences from rice and other species can be classified into two types (I and II). OsGDH1-3 belonged to type II genes, whereas OsGDH4 belonged to type I like gene. Our data implied that the expansion rate of type I genes was much slower than that of type II genes and species-specific expansion contributed to the evolution of type II genes in plants. The expression levels of the different members of GDH family in rice were evaluated using quantitative real-time PCR and microarray analysis. Gene expression patterns revealed that OsGDH1, OsGDH2, and OsGDH4 are expressed ubiquitously in various tissues, whereas OsGDH3 expression is glumes and stamens specific. The expression of the OsGDH family members responded differentially to nitrogen and phosphorus-deprivation, indicating their roles under such stress conditions. Implications of the expression patterns with respect to the functions of these genes were discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Gene Expression Profiling
  • Gene Expression Regulation, Plant
  • Glutamate Dehydrogenase / genetics
  • Glutamate Dehydrogenase / metabolism*
  • Molecular Sequence Data
  • Multigene Family*
  • Nitrogen / metabolism*
  • Oligonucleotide Array Sequence Analysis
  • Oryza / enzymology
  • Oryza / genetics*
  • Phosphorus / metabolism*
  • Phylogeny
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • RNA, Plant / genetics
  • Sequence Alignment
  • Sequence Analysis, DNA
  • Stress, Physiological

Substances

  • Plant Proteins
  • RNA, Plant
  • Phosphorus
  • Glutamate Dehydrogenase
  • Nitrogen