Abstract
Loss-of-function Aspergillus nidulans CclA, a Bre2 ortholog involved in histone H3 lysine 4 methylation, activated the expression of cryptic secondary metabolite clusters in A. nidulans. One new cluster generated monodictyphenone, emodin and emodin derivatives, whereas a second encoded two anti-osteoporosis polyketides, F9775A and F9775B. Modification of the chromatin landscape in fungal secondary metabolite clusters allows for a simple technological means to express silent fungal secondary metabolite gene clusters.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Anti-Bacterial Agents / biosynthesis
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Anti-Bacterial Agents / chemistry
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Aspergillus nidulans / enzymology
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Aspergillus nidulans / genetics*
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Aspergillus nidulans / metabolism
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Chromatin / metabolism*
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Chromatography, High Pressure Liquid
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Chromatography, Thin Layer
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Drug Discovery*
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Emodin / chemistry
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Gene Deletion
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Gene Expression Regulation, Fungal*
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Histones / metabolism
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Immunoprecipitation
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Macrolides / chemistry
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Mass Spectrometry
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Methylation
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Molecular Structure
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Multigene Family*
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Polyketide Synthases / genetics
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Polymerase Chain Reaction
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Promoter Regions, Genetic
Substances
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Anti-Bacterial Agents
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Chromatin
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Histones
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Macrolides
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Polyketide Synthases
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Emodin