Investigation of biomass and lipids production with Neochloris oleoabundans in photobioreactor

Bioresour Technol. 2009 Dec;100(23):5988-95. doi: 10.1016/j.biortech.2009.06.004. Epub 2009 Jun 26.

Abstract

The fresh water microalga Neochloris oleoabundans was investigated for its ability to accumulate lipids and especially triacylglycerols (TAG). A systematic study was conducted, from the determination of the growth medium to its characterization in an airlift photobioreactor. Without nutrient limitation, a maximal biomass areal productivity of 16.5 g m(-2) day(-1) was found. Effects of nitrogen starvation to induce lipids accumulation was next investigated. Due to initial N. oleoabundans total lipids high content (23% of dry weight), highest productivity was obtained without mineral limitation with a maximal total lipids productivity of 3.8 g m(-2) day(-1). Regarding TAG, an almost similar productivity was found whatever the protocol was: continuous production without mineral limitation (0.5 g m(-2) day(-1)) or batch production with either sudden or progressive nitrogen deprivation (0.7 g m(-2) day(-1)). The decrease in growth rate reduces the benefit of the important lipids and TAG accumulation as obtained in nitrogen starvation (37% and 18% of dry weight, respectively).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bioelectric Energy Sources
  • Biomass
  • Bioreactors*
  • Biotechnology / methods
  • Eukaryota / metabolism*
  • Industrial Microbiology / methods
  • Lipids / chemistry*
  • Nitrogen / chemistry
  • Pigmentation
  • Triglycerides / chemistry

Substances

  • Lipids
  • Triglycerides
  • Nitrogen