Abstract
Excision of staphylococcal cassette chromosome mec (SCCmec) is mediated through the ccrA- and -B-encoded recombinases. We investigated the effects of different antimicrobial agents on ccrA expression by using a ccrA::lacZ fusion and reverse transcription-PCR with methicillin (meticillin)-resistant Staphylococcus aureus strains MW2 (SCCmec IVa) and N315 (SCCmec II). Upregulation of ccrA was observed upon exposure to beta-lactam antibiotics. Vancomycin increased ccrA expression in MW2 but had no effect on N315. Vancomycin may contribute to the transfer of SCCmec IVa but have no effect in SCCmec II.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / pharmacology
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Bacterial Proteins / genetics*
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Gene Expression Regulation, Bacterial / drug effects
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Gene Expression Regulation, Bacterial / genetics
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Methicillin-Resistant Staphylococcus aureus / drug effects
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Methicillin-Resistant Staphylococcus aureus / genetics*
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Methicillin-Resistant Staphylococcus aureus / metabolism
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Nitrophenylgalactosides / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Vancomycin / pharmacology
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beta-Galactosidase / metabolism
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beta-Lactamases / genetics*
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beta-Lactams / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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beta-Lactams
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2-nitrophenylgalactoside
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Nitrophenylgalactosides
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Vancomycin
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beta-Galactosidase
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beta-Lactamases
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carbapenemase