A high-resolution radiation hybrid map of sheep chromosome X and comparison with human and cattle

Cytogenet Genome Res. 2009;125(1):40-5. doi: 10.1159/000207520. Epub 2009 Jul 14.

Abstract

A radiation hybrid (RH) map of sheep X chromosome (Ovisaries; OARX) containing 146 physically anchored loci was generated in this study, providing information for comparative X chromosome analysis between the maps of sheep, human, and cattle. Primers typed on the USUoRH5000 ovine whole-genome radiation hybrid panel were designed from sequences predicted to be on the ovine X chromosome, based on comparative mapping within the virtual sheep genome browser (v1.2). The resulting RH map for the ovine X chromosome consists of 4 linkage groups composed of 76 BAC end sequences (BES), 28 gene loci that were confirmed within ovine BAC clones in the CHORI-243 ovine BAC library, 28 additional gene loci from the ovine comparative map and 14 polymorphic sequence tagged sites (STS) from the OARX linkage map. This first-generation RH map of OARX contributes to the expansion of a comprehensive ovine genome map for sheep and provides evidence of rearrangements in loci order compared to the human and cattle orders.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle / genetics*
  • Chromosomes, Artificial, Bacterial / genetics
  • Chromosomes, Human, X / genetics*
  • Humans
  • Microsatellite Repeats
  • Radiation Hybrid Mapping / methods
  • Radiation Hybrid Mapping / veterinary*
  • Sheep / genetics*
  • Species Specificity
  • X Chromosome / genetics*