NMR assignment of the domain 513-651 from the SARS-CoV nonstructural protein nsp3

Biomol NMR Assign. 2007 Dec;1(2):191-4. doi: 10.1007/s12104-007-9052-x. Epub 2007 Oct 30.

Abstract

Sequence-specific NMR assignments of an internal domain of the protein nsp3, nsp3(513-651), which is a part of the SARS coronavirus (SARS-CoV) replicase polyprotein, have been determined, using triple-resonance NMR experiments with the uniformly [(13)C,(15)N]-labeled protein. The complete assignments (>99%) provide the basis for the ongoing three-dimensional structure determination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Weight
  • Protein Structure, Tertiary
  • Protons
  • RNA-Dependent RNA Polymerase / chemistry*
  • Viral Nonstructural Proteins / chemistry*

Substances

  • Protons
  • Viral Nonstructural Proteins
  • Nsp3 protein, SARS-CoV
  • RNA-Dependent RNA Polymerase