NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42

Biomol NMR Assign. 2007 Dec;1(2):225-7. doi: 10.1007/s12104-007-9062-8. Epub 2007 Nov 13.

Abstract

Cdc42(T35A) is an active construct of Cdc42, a Ras GTPase involved in signal transduction, containing a single-point mutation in an important effector-binding region. We determined the backbone and side chain resonance assignments of (13)C,(15)N-labelled Cdc42(T35A) from E. coli.

MeSH terms

  • Amino Acid Sequence
  • Carbon Isotopes / chemistry
  • Magnetic Resonance Spectroscopy / methods*
  • Molecular Weight
  • Mutation
  • Nitrogen Isotopes / chemistry
  • Protein Structure, Tertiary
  • Protons
  • cdc42 GTP-Binding Protein / chemistry*

Substances

  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protons
  • cdc42 GTP-Binding Protein