Granulosicoccus coccoides sp. nov., isolated from leaves of seagrass (Zostera marina)

Int J Syst Evol Microbiol. 2010 Apr;60(Pt 4):972-976. doi: 10.1099/ijs.0.013516-0. Epub 2009 Aug 6.

Abstract

A non-pigmented, motile, Gram-negative bacterium, strain Z 271(T), was isolated from the surface of leaves of the seagrass Zostera marina which was collected in Troitza Bay (Sea of Japan, Pacific Ocean). The new isolate grew between 5 degrees C and 28 degrees C and was slightly halophilic, tolerating environments containing up to 5 % (w/v) NaCl. Strain Z 271(T) was able to degrade Tweens 20, 40 and 80 and partially degrade gelatin, but was unable to degrade casein. Phosphatidylethanolamine (36.9 %) and phosphatidylglycerol (63.1 %) were the predominant phospholipids. The major fatty acids included C(18 : 1)omega7c (43.7 %), C(16 : 1)omega7c (31.1 %) and C(16 : 0) (16.8 %). The main respiratory quinone was Q-8. The DNA-DNA relatedness value of strain Z 271(T) with Granulosicoccus antarcticus IMCC3135(T) was 35 %. The G+C content of the DNA of strain Z 271(T) was 60.2 mol%. On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, strain Z 271(T) represents a novel species of the genus Granulosicoccus for which the name Granulosicoccus coccoides sp. nov. is proposed. The type strain is Z 271(T) (=KMM 6014(T)=CIP 109923(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Typing Techniques
  • Base Composition
  • DNA, Ribosomal / analysis
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Gammaproteobacteria / classification*
  • Gammaproteobacteria / genetics
  • Gammaproteobacteria / isolation & purification*
  • Gammaproteobacteria / physiology
  • Genes, rRNA
  • Genotype
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phenotype
  • Phylogeny
  • Plant Leaves / microbiology*
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Species Specificity
  • Zosteraceae / microbiology*

Substances

  • DNA, Ribosomal
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/FJ535355