Establishment and characterization of an ovarian cell line of the silkworm, Bombyx mori

Tissue Cell. 2010 Feb;42(1):42-6. doi: 10.1016/j.tice.2009.07.002. Epub 2009 Aug 7.

Abstract

A cell line BmN-SWU1 was established from the ovarian tissues of 3-day-old fourth instar Bombyx mori larvae of the 21-872nlw variety by performing primary cultures in Grace's medium supplemented with 20% fetal bovine serum (FBS). The cell line primarily consisted of short spindle cells and round cells. The frequency of cells with chromosome number 2n=56 was 80.5%; therefore, the cell line was considered to be a diploid cell line. The population-doubling time (PDT) at 45th passage line was 57.7h. This cell line was susceptible to the B. mori nuclear polyhedrovirus (BmNPV), and the median tissue culture infective dose (TCID(50)) at a cell density of 10(5) cells/ml was 16.3 OBs/ml. The transient expression efficiency of the green fluorescent protein (GFP) gene in this cell line was 54.8%. We used the BmN-SWU1 cell line to select and establish a GFP transgenic cell line.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bombyx / cytology*
  • Bombyx / physiology
  • Bombyx / virology
  • Cell Culture Techniques
  • Cell Proliferation
  • Cells, Cultured
  • Diploidy
  • Epithelial Cells / cytology*
  • Epithelial Cells / physiology
  • Epithelial Cells / virology
  • Female
  • Gene Expression Regulation / genetics
  • Genetic Vectors / genetics
  • Green Fluorescent Proteins / genetics
  • Green Fluorescent Proteins / metabolism
  • Nucleopolyhedroviruses / genetics
  • Nucleopolyhedroviruses / pathogenicity
  • Ovary / cytology*
  • Ovary / physiology
  • Ovary / virology
  • Transfection / methods

Substances

  • Green Fluorescent Proteins