Pharmacogenetic pathway analysis for determination of sunitinib-induced toxicity

J Clin Oncol. 2009 Sep 10;27(26):4406-12. doi: 10.1200/JCO.2008.21.7679. Epub 2009 Aug 10.

Abstract

Purpose: To identify genetic markers in the pharmacokinetic and pharmacodynamic pathways of sunitinib that predispose for development of toxicities: thrombocytopenia, leukopenia, mucosal inflammation, hand-foot syndrome, and any toxicity according to National Cancer Institute Common Toxicity Criteria higher than grade 2.

Patients and methods: A multicenter pharmacogenetic association study was performed in 219 patients treated with single-agent sunitinib. A total of 31 single nucleotide polymorphisms in 12 candidate genes, together with several nongenetic variants, were analyzed for a possible association with toxicity. In addition, genetic haplotypes were developed and related to toxicity.

Results: The risk for leukopenia was increased when the G allele in CYP1A1 2455A/G (odds ratio [OR], 6.24; P = .029) or the T allele in FLT3 738T/C (OR, 2.8; P = .008) were present or CAG in the NR1I3 (5719C/T, 7738A/C, 7837T/G) haplotype (OR, 1.74; P = .041) was absent. Any toxicity higher than grade 2 prevalence was increased when the T allele of vascular endothelial growth factor receptor 2 1191C/T (OR, 2.39; P = .046) or a copy of TT in the ABCG2 (-15622C/T, 1143C/T) haplotype (OR, 2.63; P = .016) were present. The risk for mucosal inflammation was increased in the presence of the G allele in CYP1A1 2455A/G (OR, 4.03; P = .021) and the prevalence of hand-foot syndrome was increased when a copy of TTT in the ABCB1 (3435C/T, 1236C/T, 2677G/T) haplotype (OR, 2.56; P = .035) was present.

Conclusion: This exploratory study suggests that polymorphisms in specific genes encoding for metabolizing enzymes, efflux transporters, and drug targets are associated with sunitinib-related toxicities. A better understanding of genetic and nongenetic determinants of sunitinib toxicity should help to optimize drug treatment in individual patients.

Publication types

  • Multicenter Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Antineoplastic Agents / adverse effects
  • Constitutive Androstane Receptor
  • Cytochrome P-450 CYP1A1 / genetics
  • Drug-Related Side Effects and Adverse Reactions / chemically induced
  • Drug-Related Side Effects and Adverse Reactions / genetics*
  • Female
  • Genetic Predisposition to Disease / genetics
  • Haplotypes
  • Humans
  • Indoles / adverse effects*
  • Indoles / pharmacokinetics
  • Leukopenia / chemically induced
  • Male
  • Middle Aged
  • Mucositis / chemically induced
  • Pharmacogenetics / methods*
  • Polymorphism, Single Nucleotide
  • Pyrroles / adverse effects*
  • Pyrroles / pharmacokinetics
  • Risk Factors
  • Sunitinib
  • Young Adult

Substances

  • Antineoplastic Agents
  • Constitutive Androstane Receptor
  • Indoles
  • NR1I3 protein, human
  • Pyrroles
  • Cytochrome P-450 CYP1A1
  • Sunitinib