pSTAT1, pSTAT3, and T-bet as markers of disease activity in chronic inflammatory demyelinating polyradiculoneuropathy

J Peripher Nerv Syst. 2009 Jun;14(2):107-17. doi: 10.1111/j.1529-8027.2009.00220.x.

Abstract

Chronic inflammatory demyelinating polyradiculoneuropathy (CIDP) is considered an auto-immune disorder. We evaluated expression of pSTAT1, T-bet, and pSTAT3 in circulating T-cells, B-cells, and monocytes and spontaneous production of interleukin-17 (IL17), interferon-gamma (IFN gamma), and interleukin-10 (IL10) by peripheral blood mononuclear cells (PBMCs) from 14 active CIDP patients compared with 6 patients with long-lasting remission and 20 controls. Active disease patients showed higher pSTAT1, T-bet, and pSTAT3 in CD4(+) T-cells than controls (p < 0.001, p = 0.0002, p = 0.0097, respectively) and remission patients (p < 0.001, p = 0.0036, p = 0.0008, respectively). pSTAT1, T-bet, and pSTAT3 were also higher in monocytes from active CIDP patients than controls (p = 0.0011, p = 0.0041, p = 0.0413, respectively) and remission patients (p = 0.0073, p = 0.0274, p = 0.0251, respectively). Moreover in CD8(+) T-cells, pSTAT3 expression was higher in active CIDP patients than in remission patients (p = 0.0345) and in controls (p = 0.0023). IL17 and IFN gamma production were significantly higher in active CIDP patients than in controls (p < 0.0395, p = 0.0010, respectively); IFN gamma levels were higher also in remission CIDP patients (p = 0.0073). IL10 levels were higher in active phase patients than in controls (p = 0.0334). Our data suggest that pSTAT1, T-bet, and pSTAT3 can be considered putative markers of disease activity and potential targets for specific therapies.

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • B-Lymphocytes / metabolism
  • Biomarkers / blood
  • Child
  • Female
  • Humans
  • Interferon-gamma / blood
  • Interferon-gamma / metabolism
  • Interleukin-10 / blood
  • Interleukin-10 / metabolism
  • Interleukin-17 / blood
  • Interleukin-17 / metabolism
  • Leukocytes, Mononuclear / metabolism
  • Male
  • Middle Aged
  • Monocytes / metabolism*
  • Phosphorylation
  • Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / blood*
  • Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / metabolism
  • STAT1 Transcription Factor / blood*
  • STAT1 Transcription Factor / metabolism
  • STAT3 Transcription Factor / blood*
  • STAT3 Transcription Factor / metabolism
  • T-Box Domain Proteins / blood*
  • T-Box Domain Proteins / metabolism
  • T-Lymphocytes / metabolism*
  • Young Adult

Substances

  • Biomarkers
  • IL10 protein, human
  • Interleukin-17
  • STAT1 Transcription Factor
  • STAT1 protein, human
  • STAT3 Transcription Factor
  • STAT3 protein, human
  • T-Box Domain Proteins
  • T-box transcription factor TBX21
  • Interleukin-10
  • Interferon-gamma