Exfoliative cytology of the oral mucosa in burning mouth syndrome: a cytomorphological and cytomorphometric analysis

Gerodontology. 2011 Mar;28(1):44-8. doi: 10.1111/j.1741-2358.2009.00319.x.

Abstract

Objective: The aim of this study was to evaluate oral epithelial cells by exfoliative cytology in burning mouth syndrome (BMS).

Material and methods: Oral smears were collected from clinically normal-appearing mucosa by liquid-based exfoliative cytology in 40 individuals (20 BMS patients and 20 healthy controls matched for age and gender) and analysed for cytological and cytomorphometric techniques.

Results: Mean values of nuclear area (NA) for experimental and control groups were, respectively, 67.52 and 55.64 μm² (p < 0.05). Cytoplasmic area (CA) showed the following mean values: 1258.0 (experimental) and 2069.0 μm² (control). Nucleus-to-cytoplasm area ratio for the experimental group was 0.07, besides the control group was 0.03 (p < 0.05). Morphologically, oral smears exhibited normal epithelial cells in both experimental and control groups. There was a significant predominance of nucleated cells of the superficial layer in the smears of BMS patients (p = 0.00001).

Conclusion: This study revealed that oral mucosa of BMS patients exhibited significant cytomorphometric changes in the oral epithelial cells. These changes probably are associated with epithelial atrophy and a deregulated maturation process that may contribute to the oral symptoms of pain and discomfort in BMS.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Aged
  • Aged, 80 and over
  • Atrophy
  • Burning Mouth Syndrome / pathology*
  • Case-Control Studies
  • Cell Nucleus / ultrastructure
  • Cell Shape
  • Coloring Agents
  • Cytodiagnosis / instrumentation
  • Cytodiagnosis / methods
  • Cytoplasm / ultrastructure
  • Epithelial Cells / pathology
  • Female
  • Humans
  • Image Processing, Computer-Assisted / methods
  • Male
  • Middle Aged
  • Mouth Mucosa / pathology*
  • Tongue / pathology

Substances

  • Coloring Agents