Assisted assembly: how to improve a de novo genome assembly by using related species

Genome Biol. 2009;10(8):R88. doi: 10.1186/gb-2009-10-8-r88. Epub 2009 Aug 27.

Abstract

We describe a new assembly algorithm, where a genome assembly with low sequence coverage, either throughout the genome or locally, due to cloning bias, is considerably improved through an assisting process via a related genome. We show that the information provided by aligning the whole-genome shotgun reads of the target against a reference genome can be used to substantially improve the quality of the resulting assembly.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms*
  • Animals
  • Computational Biology / methods*
  • Genome*
  • Humans
  • Mammals / genetics
  • Plasmodium / genetics