Multiple cytokine profile in plasma and amniotic fluid in a mouse model of pre-term labor

Am J Reprod Immunol. 2009 Nov;62(5):339-47. doi: 10.1111/j.1600-0897.2009.00743.x.

Abstract

Problem: The rate of pre-term birth in the United States continues to rise despite several interventions. Induction of pro-inflammatory cytokines and chemokines has been implicated in the activation of the cascade of events resulting in pre-term labor. To date, no comprehensive panel of the cytokine profile in PTL has been published.

Method of study: To address cytokine profiles in pre-term labor, levels of 19 plasma and amniotic fluid cytokines were measured using a multiplex immunoassay in an inflammation-induced murine model of pre-term labor.

Results: Pro-inflammatory mediators, RANTES, KC, IL-6, and IL-12p40 were increased by 3 hr and remained high at 15 hr. Concentrations of KC, IL-6, IL-1beta, and MIP-1alpha were increased in the amniotic fluid at 15 hr. Plasma levels of anti-inflammatory mediators IL-10 and IL-13 at 15 hr were unchanged and decreased respectively.

Conclusion: These results suggest that stimulation of several pro-inflammatory cytokines occurs very early in the cascade of events and remains increased, whereas anti-inflammatory cytokines are either unchanged or decreased until the onset of delivery in an inflammation-induced mouse model of pre-term labor.

MeSH terms

  • Amniotic Fluid / immunology
  • Amniotic Fluid / metabolism*
  • Animals
  • Biomarkers / blood
  • Cytokines / biosynthesis
  • Cytokines / blood
  • Cytokines / genetics
  • Cytokines / immunology*
  • Disease Models, Animal
  • Humans
  • Immunization
  • Inflammation
  • Lipopolysaccharides / administration & dosage
  • Mice
  • Premature Birth / blood
  • Premature Birth / diagnosis
  • Premature Birth / immunology*
  • Prognosis
  • Risk Factors

Substances

  • Biomarkers
  • Cytokines
  • Lipopolysaccharides