Oligodendroglial and pan-neural crest expression of Cre recombinase directed by Sox10 enhancer

Genesis. 2009 Nov;47(11):765-70. doi: 10.1002/dvg.20559.

Abstract

Utilizing a recently identified Sox10 distal enhancer directing Cre expression, we report S4F:Cre, a transgenic mouse line capable of inducing recombination in oligodendroglia and all examined neural crest derived tissues. Assayed using R26R:LacZ reporter mice expression was detected in neural crest derived tissues including the forming facial skeleton, dorsal root ganglia, sympathetic ganglia, enteric nervous system, aortae, and melanoblasts, consistent with Sox10 expression. LacZ reporter expression was also detected in non-neural crest derived tissues including the oligodendrocytes and the ventral neural tube. This line provides appreciable differences in Cre expression pattern from other transgenic mouse lines that mark neural crest populations, including additional populations defined by the expression of other SoxE proteins. The S4F:Cre transgenic line will thus serve as a powerful tool for lineage tracing, gene function characterization, and genome manipulation in these populations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA Primers
  • Enhancer Elements, Genetic*
  • Integrases / genetics*
  • Mice
  • Mice, Transgenic
  • Neural Crest / enzymology*
  • Oligodendroglia
  • SOXE Transcription Factors / genetics*

Substances

  • DNA Primers
  • SOXE Transcription Factors
  • Sox10 protein, mouse
  • Cre recombinase
  • Integrases