In vitro free radical scavenging activity of platinum nanoparticles

Nanotechnology. 2009 Nov 11;20(45):455105. doi: 10.1088/0957-4484/20/45/455105. Epub 2009 Oct 16.

Abstract

A polyacrylic acid (PAA)-protected platinum nanoparticle species (PAA-Pt) was prepared by alcohol reduction of hexachloroplatinate. The PAA-Pt nanoparticles were well dispersed and homogeneous in size with an average diameter of 2.0 +/- 0.4 nm (n = 200). We used electron spin resonance to quantify the residual peroxyl radical ([Formula: see text]) generated from 2,2-azobis (2-aminopropane) dihydrochloride (AAPH) by thermal decomposition in the presence of O(2) and a spectrophotometric method to quantify the residual 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. PAA-Pt scavenged these two radicals in a dose-dependent manner. Platinum was the functional component. PAA-Pt reduced the rate of oxygen consumption required for linoleic acid peroxidation initiated by [Formula: see text] generated from AAPH, indicating inhibition of the propagation of linolate peroxidation. A thiobarbituric acid test also revealed dose-dependent inhibition of the linolate peroxidation by PAA-Pt. Fifty micromolar platinum, as PAA-Pt, completely quenched 250 microM DPPH radical for 5 min. Even when twice diluted in half, the PAA-Pt still quenched 100% of the 250 microM DPPH radical. The scavenging activity of PAA-Pt is durable. These observations suggest that PAA-Pt is an efficient scavenger of free radicals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amidines / chemistry
  • Biphenyl Compounds / chemistry
  • Free Radical Scavengers / chemistry*
  • Free Radical Scavengers / pharmacology*
  • Linoleic Acid / chemistry
  • Lipid Peroxidation / drug effects
  • Metal Nanoparticles / chemistry*
  • Peroxides / chemistry
  • Picrates / chemistry
  • Platinum / chemistry*

Substances

  • Amidines
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Peroxides
  • Picrates
  • perhydroxyl radical
  • Platinum
  • 2,2'-azobis(2-amidinopropane)
  • Linoleic Acid
  • 1,1-diphenyl-2-picrylhydrazyl