Rhodopsin: the functional significance of asn-linked glycosylation and other post-translational modifications

Ophthalmic Genet. 2009 Sep;30(3):109-20. doi: 10.1080/13816810902962405.

Abstract

Rhodopsin, the G-protein coupled receptor in retinal rod photoreceptors, is a highly conserved protein that undergoes several types of post-translational modifications. These modifications are essential to maintain the protein's structure as well as its proper function in the visual transduction cycle. Rhodopsin is N-glycosylated at Asn-2 and Asn-15 in its extracellular N-terminal domain. Mutations within the glycosylation consensus sequences of rhodopsin cause autosomal dominant retinitis pigmentosa, a disease that leads to blindness. Several groups have studied the role of rhodopsin's N-linked glycan chains in protein structure and function using a variety of approaches. These include the generation of a transgenic mouse model, study of a naturally occurring mutant animal model, in vivo pharmacological inhibition of glycosylation, and in vitro analyses using transfected COS-1 cells. These studies have provided insights into the possible role of rhodopsin glycosylation, but have yielded conflicting results.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Asparagine / chemistry*
  • Glycosylation
  • Humans
  • Mice
  • Molecular Sequence Data
  • Protein Processing, Post-Translational*
  • Rhodopsin / chemistry
  • Rhodopsin / physiology*
  • Sequence Homology, Amino Acid

Substances

  • Asparagine
  • Rhodopsin