The numbers of lower limb amputees participating in recreational activity date back more than 25 years. Previous studies have shown that 60% of lower limb amputees participated in recreational activities, including sports. To date, research in The Netherlands into sports participation of this specific amputee population is insufficient. The purpose of the reported survey was to investigate the sports participation habits of lower limb amputees in the Province of Drenthe, The Netherlands, using a self-constructed questionnaire. A total of 105 lower limb amputees responded (36%), a large proportion of whom were traumatic amputees (31%). Of the respondents, 34 (32%) participated in some form of sport. Results indicated that participation in sport before the amputation was a predetermining factor for amputees to participate in sports whilst the level of amputation, age and etiology were not predetermining factors of participation in sports after a lower limb amputation.